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Characterization of Commercial Pectin Preparations by Spectroscopic and Chromatographic Techniques.

Pectin has a long history as a food additive. However, elucidation of its fine structural and property relationships remains elusive. Recent research has focused on pectin's ability to complex with divalent heavy metals to aid in characterizing it. Commercial pectins of unknown composition were obtained from local grocers. Purified pectin samples from orange peel, lemon peel, and apple pomace, each of low and high levels of methyl esterification and of unknown distribution pattern were also purchased. Instead of metal complexation, several highly absorbing dyes such as Ruthenium Red, Nile Blue, and Acridine Orange were used to complex with the pectins and their resulting UV-Vis spectral patterns were employed to determine if one can characterize the different pectins. Chemometric methods are also included to aid in distinguishing them apart.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-3262
Date03 May 2008
CreatorsDixon, Daniel Wayne
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceElectronic Theses and Dissertations
RightsCopyright by the authors.

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