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Purification and Characterization of a Differentiation Factor From Rat Lung Conditioned Medium

A Differentiation Factor (DF) was purified from rat lung conditioned medium by a four-steps procedure. The DF has a molecular weight of 27000, and an isoelectric point of 4.70. Although DF is stable up to 60°C, it is sensitive to digestion by trypsin, chymotrypsin and subtilisin. DF forms granulocyte colonies in soft agar. Studies using anti-NRK CSF antibody demonstrated that DF is distinct from GM-CSF.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798062
Date05 1900
CreatorsAnsari, Naser A. (Naser Awni)
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvii, 73 leaves : ill., Text
RightsPublic, Ansari, Naser A. (Naser Awni), Copyright, Copyright is held by the author, unless otherwise noted. All rights

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