by Long Wan Wong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 77-93. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iii / LIST OF TABLES --- p.vii / LIST OF FIGURES --- p.viii / LIST OF ABBREVIATIONS --- p.ix / INTRODUCTION --- p.1 / ANATOMY OF OLFACTORY MUCOSA --- p.1 / NEUROGENESIS AND DIFFERENTIATION OF OLFACTORY RECEPTOR NEURONS --- p.3 / ANATOMY AND DEVELOPMENT OF THE NERVE FIBER LAYER OF THE OLFACTORY BULB --- p.4 / FACTORS INFLUENCING NEURONAL DIFFERENTIATION --- p.6 / Chapter (1) --- Extracellular Matrix Molecules / Chapter (2) --- Cell Adhesion Molecules / Chapter (3) --- Soluble Diffusible Molecules / ASTROCYTES AS A SUBSTRATUM FOR CELL CULTURE --- p.12 / EXPERIMENTAL PURPOSE AND DESIGN --- p.15 / MATERIALS AND METHODS --- p.16 / PREPARATION OF CELLULAR SUBSTRATA --- p.16 / Chapter (1) --- Cortical Astrocyte Monolayers / Chapter (2) --- OB Astrocyte Monolayers / Chapter (3) --- ONL Glial Cell Monolayers / Chapter (4) --- Skin Fibroblast Monolayers / DISSOCIATION OF OLFACTORY MUCOSA --- p.22 / TRANSMISSION AND SCANNING ELECTRON MICROSCOPY --- p.23 / IMMUNOFLUORESCENCE --- p.24 / IMMUNOHISTOCHEMISTRY --- p.26 / COUNTING OF ORNs BEARING NEURITES AND MEASUREMENT OF NEURITE LENGTH --- p.26 / ANALYSIS OF CONDITIONED MEDIA --- p.27 / Chapter (1) --- Preparation of Conditioned Media / Chapter (2) --- Ultrafiltration / Chapter (3) --- Heat Treatment / Chapter (4) --- Protein Assay / Chapter (5) --- Investigation of Concentration-Activity Relationship / TABLE 1 --- p.30 / FIGURES 1-2 --- p.31 / RESULTS --- p.36 / TRANSMISSION AND SCANNING ELECTRON MICROSCOPY --- p.36 / Chapter (1) --- Cortical Astrocytes / Chapter (2) --- ORNs on Cortical Astrocytes / "OLFACTORY NEURITE EXTENSION ON CORTICAL ASTROCYTES, OB ASTROCYTES, ONL GLIAL CELLS, AND FIBROBLASTS" --- p.38 / Chapter (1) --- Morphology of the Cellular Substrata / Chapter (2) --- Morphology of ORNs on Various Cellular Substrata / Chapter (3) --- Quantitative Studies of Olfactory Neurite Extension on the Cellular Substrata / CELL ADHESION MOLECULES ON ASTROCYTES --- p.41 / INFLUENCE OF SOLUBLE SUBSTANCES ON OLFACTORY NEURITE EXTENSION --- p.42 / ANALYSIS OF MEDIA CONDITIONED BY FIBROBLASTS --- p.44 / Chapter (1) --- Molecular Weight Range of the Inhibitory Substance(s) / Chapter (2) --- Culture of ORNs on Cortical Astrocytes in Heat-treated CMF / Chapter (3) --- Protein Assay / Chapter (4) --- Concentration-Activity Relationship / TABLES 2-8 --- p.46 / FIGURES 3-9 --- p.53 / DISCUSSION --- p.69 / CONCLUSIONS --- p.76 / REFERENCES --- p.77
Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318824 |
Date | January 1991 |
Contributors | Wong, Long Wan., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences. |
Publisher | Chinese University of Hong Kong |
Source Sets | The Chinese University of Hong Kong |
Language | English |
Detected Language | English |
Type | Text, bibliography |
Format | print, x, 93 leaves : ill. ; 30 cm. |
Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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