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Cell culture studies of olfactory receptor neurons.

by Long Wan Wong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 77-93. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iii / LIST OF TABLES --- p.vii / LIST OF FIGURES --- p.viii / LIST OF ABBREVIATIONS --- p.ix / INTRODUCTION --- p.1 / ANATOMY OF OLFACTORY MUCOSA --- p.1 / NEUROGENESIS AND DIFFERENTIATION OF OLFACTORY RECEPTOR NEURONS --- p.3 / ANATOMY AND DEVELOPMENT OF THE NERVE FIBER LAYER OF THE OLFACTORY BULB --- p.4 / FACTORS INFLUENCING NEURONAL DIFFERENTIATION --- p.6 / Chapter (1) --- Extracellular Matrix Molecules / Chapter (2) --- Cell Adhesion Molecules / Chapter (3) --- Soluble Diffusible Molecules / ASTROCYTES AS A SUBSTRATUM FOR CELL CULTURE --- p.12 / EXPERIMENTAL PURPOSE AND DESIGN --- p.15 / MATERIALS AND METHODS --- p.16 / PREPARATION OF CELLULAR SUBSTRATA --- p.16 / Chapter (1) --- Cortical Astrocyte Monolayers / Chapter (2) --- OB Astrocyte Monolayers / Chapter (3) --- ONL Glial Cell Monolayers / Chapter (4) --- Skin Fibroblast Monolayers / DISSOCIATION OF OLFACTORY MUCOSA --- p.22 / TRANSMISSION AND SCANNING ELECTRON MICROSCOPY --- p.23 / IMMUNOFLUORESCENCE --- p.24 / IMMUNOHISTOCHEMISTRY --- p.26 / COUNTING OF ORNs BEARING NEURITES AND MEASUREMENT OF NEURITE LENGTH --- p.26 / ANALYSIS OF CONDITIONED MEDIA --- p.27 / Chapter (1) --- Preparation of Conditioned Media / Chapter (2) --- Ultrafiltration / Chapter (3) --- Heat Treatment / Chapter (4) --- Protein Assay / Chapter (5) --- Investigation of Concentration-Activity Relationship / TABLE 1 --- p.30 / FIGURES 1-2 --- p.31 / RESULTS --- p.36 / TRANSMISSION AND SCANNING ELECTRON MICROSCOPY --- p.36 / Chapter (1) --- Cortical Astrocytes / Chapter (2) --- ORNs on Cortical Astrocytes / "OLFACTORY NEURITE EXTENSION ON CORTICAL ASTROCYTES, OB ASTROCYTES, ONL GLIAL CELLS, AND FIBROBLASTS" --- p.38 / Chapter (1) --- Morphology of the Cellular Substrata / Chapter (2) --- Morphology of ORNs on Various Cellular Substrata / Chapter (3) --- Quantitative Studies of Olfactory Neurite Extension on the Cellular Substrata / CELL ADHESION MOLECULES ON ASTROCYTES --- p.41 / INFLUENCE OF SOLUBLE SUBSTANCES ON OLFACTORY NEURITE EXTENSION --- p.42 / ANALYSIS OF MEDIA CONDITIONED BY FIBROBLASTS --- p.44 / Chapter (1) --- Molecular Weight Range of the Inhibitory Substance(s) / Chapter (2) --- Culture of ORNs on Cortical Astrocytes in Heat-treated CMF / Chapter (3) --- Protein Assay / Chapter (4) --- Concentration-Activity Relationship / TABLES 2-8 --- p.46 / FIGURES 3-9 --- p.53 / DISCUSSION --- p.69 / CONCLUSIONS --- p.76 / REFERENCES --- p.77

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318824
Date January 1991
ContributorsWong, Long Wan., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 93 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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