By differential screening of a cDNA library of the resurrection plant Xerophyta viscosa, a cDNA termed XvPer I was isolated for its over-expression under dehydration stress. Analysis of the cDNA sequence indicated a hydrophilic protein of a predicted Mw of 24.2 kDa with high identity to plant l-Cys peroxiredoxins, a novel antioxidant enzyme. Southern blot analysis revealed that XvPer I was most probably a single copy gene. The polypeptide sequence had significant identities (-70%) with other recently identified plant I-Cys peroxiredoxins with an absolutely conserved active site (PVCTTE). The protein sequence also had a putative bipartite nuclear localization signal.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/6728 |
| Date | January 2005 |
| Creators | Mowla, Shaheen B |
| Contributors | Mundree, Sagadevan G, Thomson, Jennifer Ann, Farrant, Jill M, Foyer, C H, Theodoulou, F |
| Publisher | University of Cape Town, Faculty of Science, Department of Molecular and Cell Biology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | application/pdf |
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