Using cultured Y-1 mouse adrenal tumor cells which produce the steroid 20(-hydroxypregn-4-en-3-one (20-DHP), it was found that 10-5 M corticosterone and deoxycorticosterone increased basal and inhibited ACTH-induced 20-DHP production. The steroid effects were concentration-dependent, reversible, and specific since six other steroids did not stimulate steroidogenesis and varied in their ability to inhibit ACTH-induced steroidogenesis. Cytochalasin D inhibited steroid-stimulated 20-DHP production, suggesting a mechanism of steroid stimulation similar to that of ACTH. Steroidogenesis stimulated by cholera toxin, (Bu) 2 cAMP, or pregnenolone was not inhibited by exogenous steroid; corticosterone increased basal and inhibited ACTH-induced intracellular cAMP production. Steroids altered cell surface morphology. These findings suggest that steroids alter adrenal steroidogenesis by acting within the plasma membrane.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc504128 |
| Date | 08 1900 |
| Creators | Mattson, Mark Paul |
| Contributors | Mrotek, James J., Busbee, David L. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | v, 45 leaves: ill., Text |
| Rights | Public, Mattson, Mark Paul, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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