Wu-shun, Felix Wong. / Thesis (M.D.)--Chinese University of Hong Kong, 1991. / Includes bibliographies. / Title page --- p.i / Table of contents --- p.ii / Acknowledgements --- p.vi / Abstract --- p.viii / Declaration --- p.xiii / List of abbreviation --- p.xiv / Chapter Chapter one： --- Introduction --- p.1 / Chapter 1.1 --- Overview --- p.2 / Chapter 1.2 --- Aims of the study --- p.5 / Chapter Chapter two： --- Literature review --- p.8 / Chapter 2.1 --- Cell cycle and tumour growth --- p.9 / Chapter 2.1.1 --- Cell cycle --- p.9 / Chapter 2.1.2 --- Tumour growth --- p.14 / Chapter 2.2 --- Kinetic studies --- p.21 / Chapter 2.2.1 --- Radioisotopic studies --- p.21 / Chapter 2.2.2 --- Flow cytometry --- p.28 / Chapter 2.2.3 --- Monoclonal antibody --- p.32 / Chapter 2.3 --- Monoclonal antibody Ki-67 --- p.39 / Chapter 2.3.1 --- Development of Ki-67 --- p.39 / Chapter 2.3.2 --- The nature of the Ki-67 antigen --- p.42 / Chapter 2.3.3 --- Comparison with other kinetic methods --- p.45 / Chapter 2.3.4 --- Reported studies --- p.50 / Chapter 2.4 --- immunocytochemical staining --- p.63 / Chapter 2.4.1 --- Principle of immunostaining --- p.63 / Chapter 2.4.2 --- Fixation and processing methods --- p.69 / Chapter Chapter three： --- Materials and methods --- p.75 / Chapter 3.1 --- Cell culture --- p.76 / Chapter 3.1.1 --- Culture medium --- p.76 / Chapter 3.1.2 --- Origin and maintenance of cell line --- p.76 / Chapter 3.1.3 --- Coversip monolayer culture --- p.80 / Chapter 3.1.4 --- Multicellular spheroid culture --- p.80 / Chapter 3.1.5 --- Growth curve study --- p.81 / Chapter 3.1.6 --- Cytocentrifuge slide preparation --- p.81 / Chapter 3.2 --- immunoperoxidase staining --- p.83 / Chapter 3.2.1 --- Materials of immunoperoxidase staining --- p.83 / Chapter 3.2.2 --- Immunoperoxidase staining method --- p.86 / Chapter 3.3 --- Cell counting method --- p.92 / Chapter 3.3.1 --- Interactive cell counting system --- p.92 / Chapter 3.3.2 --- Cell counting methods --- p.95 / Chapter Chapter four： --- Proliferative activities of tumour cells IN VITRO --- p.104 / Chapter 4.1 --- Identification of cell proliferation of B16 melanoma cellsin VITRO --- p.105 / Chapter 4.1.1. --- Materials and methods --- p.106 / Chapter 4.1.2. --- Results --- p.107 / Chapter 4.1.3 --- Discussion --- p.110 / Chapter 4.2 --- Staining patterns of proliferating OCC1 cells in vitro --- p.117 / Chapter 4.2.1 --- Materials and methods --- p.117 / Chapter 4.2.2 --- Results --- p.118 / Chapter 4.2.3 --- Discussion --- p.121 / Chapter 4.3 --- "Comparative in vitro studies of cell proliferation using AgNOR counts, anti-BrdU, AD203 and Ki-67" --- p.130 / Chapter 4.3.1. --- Materials and methods --- p.130 / Chapter 4.3.2. --- Results --- p.131 / Chapter 4.3.3 --- Discussion --- p.134 / Chapter 4.4 --- Proliferative activities of tumor cells in vitro --- p.139 / Chapter 4.4.1. --- Materials and methods --- p.140 / Chapter 4.4.2. --- Results --- p.141 / Chapter 4.4.3 --- Discussion --- p.146 / Chapter Chapter five： --- Growth fraction in human genital tissues --- p.156 / Chapter 5.1 --- Cell proliferation in normal and neoplastic cervical tissues --- p.157 / Chapter 5.1.1. --- Materials and methods --- p.158 / Chapter 5.1.2. --- Results --- p.161 / Chapter 5.1.3 --- Discussion --- p.154 / Chapter 5.2 --- Tumour growth fraction in cervical carcinoma --- p.172 / Chapter 5.2.1. --- Materials and methods --- p.172 / Chapter 5.2.2. --- Results --- p.173 / Chapter 5.2.3 --- Discussion --- p.177 / Chapter 5.3 --- Tumour growth fraction in ovarian carcinoma --- p.185 / Chapter 5.3.1. --- Materials and methods --- p.185 / Chapter 5.3.2. --- Results --- p.186 / Chapter 5.3.3 --- Discussion --- p.190 / Chapter Chapter six： --- Conclusion --- p.198 / Chapter 6.1 --- Overview and future work --- p.199 / Chapter 6.2 --- Conclusion --- p.211 / references --- p.213 / Appendix： --- p.246 / Chapter (A) --- Additional Experiments / Chapter Experiment 1 --- Highest selection counting method --- p.246 / Chapter Experiment 2 --- Double staining of B16 melanoma cells --- p.248 / Chapter Experiment 3 --- Trypan blue exclusion test for viability --- p.250 / Chapter (B) --- Selected publications by the author / Chapter Publication 1 --- Characteristics of a cell line established from a Chinese patient with a squamous carcinoma of the uterine cervix --- p.252 / Chapter Publication 2 --- Establishment and characterization of a new human cell line derived from ovarian clear cell carcinoma --- p.258 / Chapter Publication 3 --- "Identification of ""non-proliferating"" B16 melanoma cells using monoclonal antibody (AD203) against the Ml subunit of ribonucleotide reductase" --- p.267 / Chapter Publication 4 --- The correlation of agyrophilic nucleolar organiser regions (AgNORs) count to bromodeoxyuridine incorporation and Ki-67 scores in an ovarian carcinoma cell line --- p.275 / Chapter Publication 5 --- Immunohistochemical determination of tumour growth fraction in human ovarian carcinoma --- p.278 / Chapter Publication 6 --- Tumor growth fraction in cervical carcinoma --- p.283
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318818 |
| Date | January 1991 |
| Contributors | Wong, Wu-shun., Chinese University of Hong Kong Graduate School. Division of Medicine. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | print, xiv, 289 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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