Primary cultures of stromal-vascular (S-V) cells from adipose tissue were
used to investigate the regulation of preadipocyte development. Differentiation of
S-V cells was found to be under hormonal control. Insulin and glucocorticoids are
essential for S-V cell differentiation in culture.
S-V cells from both newborn and mature pig adipose tissue and sera from
both ages were used to examine the effect of age on preadipocyte development.
S-V cells from newborn pigs replicated faster and appeared more responsive to
serum borne factors influencing S-V cell growth and development in culture. Serum
source (newborn vs mature) did not affect differentiation of S-V cells from newborn
or mature pig adipose tissue.
When sera from fed or fasted pigs were used to culture newborn pig S-V
cells, fasted pig sera stimulated greater differentiation and decreased cell replication
as indicated by DNA content of rat S-V cell culture.
Lean pig serum compared to obese pig serum, increased differentiation
activity in culture of S-V cells an effect which may be influenced by sex.
When sera from rat and pig were subjected to gel filtration fractionation on
Sephacryl S-200 column, the elution profiles of both sera were similar. Rat serum
contained six additional peaks (280 nm) not present in pig serum. Rat serum
fraction two (apparent molecular size 67-150 kD) promoted greater differentiation
of S-V cells than other rat serum fractions or pig serum fraction two. Fraction three
(apparent molecular size 17-43 kD) of both sera inhibited differentiation and lipid
filling in cultures of S-V cells but only rat fraction three promoted cell proliferation.
Rat and pig S-V cells have different morphology when differentiated.
Differentiated rat S-V cells appeared as individual cells when cultured in serum free
or serum supplemented medium while differentiated pig S-V cells appeared as
individual cells in serum free medium and as a tight cluster of cells in serum
supplemented medium. Both cells responded differently to sera obtained from pigs
of differing ages and development of rat S-V cells was influenced by anatomic site. / Graduation date: 1992
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/36876 |
| Date | 16 March 1992 |
| Creators | Akanbi, Kamil Agbolade |
| Contributors | Hu, Ching Yuan |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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