Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2018 / Cataloged from PDF version of thesis. / Includes bibliographical references (pages 143-151). / DNA is a highly programmable molecule that can be designed to self-assemble into nearly arbitrary 2D and 3D nanoscale structures. DNA origami is a particularly versatile method to achieve complex molecular architectures. However, the rules for designing scaffolded DNA origami have not been well-formalized, which hinders both the investigation of characteristics of well- and poorly-folded structures as well as the participation of a larger scientific audience in DNA nanotechnology. In my thesis work, a fully automatic inverse design procedure DAEDALUS (DNA Origami Sequence Design Algorithm for User-defined Structures) has been developed that programs arbitrary wireframe DNA assemblies based on an input wireframe mesh without reliance on user feedback. This general, top-down strategy is able to design nearly arbitrary DNA architectures, routing the scaffold strand using a spanning tree algorithm and adding staple strands in a prescribed manner. The wireframe nanoparticles produced can use antiparallel crossover (DX) motifs, for robust selfassembly, parallel paranemic crossover (PX) motifs, for staple-free self-assembly, or a hybrid of the two, to minimize the number of staples required for folding to the ones necessary for functionalization. The thermodynamics of the self-assembly of these wireframe structures, and the effects of scaffold and staple routing, are investigated using quantitative PCR and FRET measurements, tracking fluorescence to elucidate global and local folding events. The framework developed should enable the broad participation of nonexperts in this powerful molecular design paradigm and set the foundation for further predictive models of DNA self-assembly. / by Sakul Ratanalert. / Ph. D. / Ph.D. Massachusetts Institute of Technology, Department of Chemical Engineering
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/121818 |
| Date | January 2018 |
| Creators | Ratanalert, Sakul. |
| Contributors | Mark Bathe and Gregory C. Rutledge., Massachusetts Institute of Technology. Department of Chemical Engineering., Massachusetts Institute of Technology. Department of Chemical Engineering |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 151 pages, application/pdf |
| Rights | MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission., http://dspace.mit.edu/handle/1721.1/7582 |
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