The "modified triester" and phosphodichloridite procedures for oligoribonucleotide synthesis, using 2'- silylated nucleosides, were compared. Phosphodichloridite reagents were faster and more efficient than the arylsulphonyl reagents for both dinucleotide and mononucleotide phosphotriester synthesis. / The trichloroethyl group was better than tribromoethyl, p-nitrophenethyl or methyl protecting groups for solution-phase synthesis. Trichloroethyl dichlorophosphite was used in block condensations to prepare CCCGA and UCAUAA. / An automated RNA synthesizer was developed. Nucleoside methyl chlorophosphites were used with silica gel supports. Polystyrene, polyacryloylmorpholide and controlled pore glass supports were also used but were not as satisfactory. Oligoribonucleotides, up to 17 units long, were rapidly prepared in high overall yield. The sequences were purified by TLC and gel electrophoresis. Enzymatic degradations and HPLC confirmed the composition of the products.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71919 |
| Date | January 1984 |
| Creators | Pon, Richard T. (Richard Timothy) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000194591, proquestno: AAINK66661, Theses scanned by UMI/ProQuest. |
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