The present study was focused to develop an analytical method to analyse â-lactam antibiotics present in aqueous solutions and able to be applied directly in the fields. The method here presented is based on the chemical reaction between â-lactam antibiotics with phosphomolybdic acid, a heteropolyacid able to be reduced by the thiols obtained after the acid hydrolysis of the â-lactam antibiotics. The blue product obtained, which is proportional to the antibiotic amount, is adsorbed on polyurethane foam, and the intensity of color is directly evaluated on the solid material by means of diffuse reflectance spectroscopy. For all six evaluated â-lactam antibiotics (amoxicillin, ampicillin, penicillin V, penicillin G, cefotaxime, cefuroxime), the analytical parameters achieved were: LOD = 0.03 µg/ml, LOQ = 0.1 µg/ml, RSD = 4.0 %. The study of possible interferences showed that using solid phase extraction is possible to eliminate even strong interferences as ascorbic acid, cysteine and reducing salts like stannous chloride. The present method has avoided, as much as possible, the use of expensive and contaminating solvents and chemical reagents. The method presents the possibility to be applied using only a desktop scanner and a personal PC for measuring the color of the adsorbed material on the PUF.
| Identifer | oai:union.ndltd.org:DUETT/oai:DUETT:duett-01052006-142652 |
| Date | 12 January 2006 |
| Creators | Rodriguez, Roman |
| Contributors | Evelin Denkhaus, Heinz-Martin Kuss |
| Publisher | Gerhard-Mercator-Universitaet Duisburg |
| Source Sets | Dissertations and other Documents of the Gerhard-Mercator-University Duisburg |
| Language | German |
| Detected Language | English |
| Type | text |
| Format | application/pdf, text/html |
| Source | http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-01052006-142652/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. Hiermit erteile ich der Universitaet Duisburg das nicht-ausschliessliche Recht unter den unten angegebenen Bedingungen, meine Dissertation, Staatsexamens- oder Diplomarbeit, meinen Forschungs- oder Projektbericht zu veroeffentlichen und zu archivieren. Ich behalte das Urheberrecht und das Recht das Dokument zu veroeffentlichen und in anderen Arbeiten weiterzuverwenden. |
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