The active site of the NiFe-hydrogenase from <i>Desulfovibrio vulgaris</i> Miyazaki F was investigated by means of X-ray Absorption Spectroscopy (XAS). Using the Extended X-ray Absorption Fine Structure (EXAFS) the active site was structurally characterized to determine differences between four different catalytic states of the enzyme. The nature of an additional ligand which is present in the oxidized forms of the enzyme was revealed and the distance between both metal atoms of the heterobinuclear site was calculated. Suitable data analyzation techniques were developed using simulated spectra. For the verification of these techniques, spectra from model compounds structurally characterized by other methods were used. To ensure sufficient quality of the spectra a newly developed quality control system using statistical criteria was employed.
| Identifer | oai:union.ndltd.org:DUETT/oai:DUETT:duett-02182005-093235 |
| Date | 18 February 2005 |
| Creators | Lippold, Björn |
| Contributors | Prof. Dr. Günter Geismar, Prof. Dr. Gerald Henkel |
| Publisher | Gerhard-Mercator-Universitaet Duisburg |
| Source Sets | Dissertations and other Documents of the Gerhard-Mercator-University Duisburg |
| Language | German |
| Detected Language | English |
| Type | text |
| Format | application/octet-stream, application/pdf, text/html |
| Source | http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-02182005-093235/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. Hiermit erteile ich der Universitaet Duisburg das nicht-ausschliessliche Recht unter den unten angegebenen Bedingungen, meine Dissertation, Staatsexamens- oder Diplomarbeit, meinen Forschungs- oder Projektbericht zu veroeffentlichen und zu archivieren. Ich behalte das Urheberrecht und das Recht das Dokument zu veroeffentlichen und in anderen Arbeiten weiterzuverwenden. |
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