The protein Myoglobin was used as matix-molecule to produce monomeric porphyrins in solution. The protein complexes of differen zinc pheophorbides and zinc protoporphyrin have been studied by UV/Vis and NMR spectroscopy. The protein stability versus unfolding and NMR results Based on quantum mechanical calculations analysis of EPR- and ENDOR-spectra of the light excited triplet state yielded identification of α-protons and methyl β-protons have been identified for the first time. The data acquired agrees well with previously published data on native reaction centers. The terminal electron acceptors, [4Fe4S] centers FA and FB in Photosystem I (PSI), have been modelled by peptides with 16 amino acid length synthezied by SPPS Fmoc strategy. Both peptides incorporated a [4Fe4S] cluster in oxidation state 2 /1 as prooven by UV/Vis, EPR and Mössbauer spectroscopy. The redoxpotential for the one electron reduction was found to be -470 mV for both modell peptides.
| Identifer | oai:union.ndltd.org:DUETT/oai:DUETT:duett-06092006-133531 |
| Date | 14 June 2006 |
| Creators | Breitenstein, Christoph |
| Contributors | Prof. Wiebran S. Veeman, Prof. Wolfgang Gärtner |
| Publisher | Gerhard-Mercator-Universitaet Duisburg |
| Source Sets | Dissertations and other Documents of the Gerhard-Mercator-University Duisburg |
| Language | German |
| Detected Language | English |
| Type | text |
| Format | application/pdf, text/html |
| Source | http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-06092006-133531/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. Hiermit erteile ich der Universitaet Duisburg das nicht-ausschliessliche Recht unter den unten angegebenen Bedingungen, meine Dissertation, Staatsexamens- oder Diplomarbeit, meinen Forschungs- oder Projektbericht zu veroeffentlichen und zu archivieren. Ich behalte das Urheberrecht und das Recht das Dokument zu veroeffentlichen und in anderen Arbeiten weiterzuverwenden. |
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