M.S. / Environmental Science / A technique to measure arsenobetaine and arsenocholine in fish tissue by fast atom bombardment mass spectrometry was developed with particular attention to quantitative analysis. Experiments were performed which demonstrate analysis of the compounds desorbed directly from thin layer silica chromatography matrices, quantitative analysis of arsenobetaine in real fish samples, and accurate mass measurement of arsenobetaine in normal FAB/MS using peaks from the glycerol matrix as mass references. Improvements to the technique to quantitatively measure these important arsenic metabolites are suggested including optimization of the extraction/isolation procedures and use of isotopically labelled internal standards or surrogates for more accurate measurements.
| Identifer | oai:union.ndltd.org:OREGON/oai:content.ohsu.edu:etd/176 |
| Date | 08 1900 |
| Creators | Zimmerman, Michael L. |
| Publisher | Oregon Health & Science University |
| Source Sets | Oregon Health and Science Univ. Library |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | Needs Adobe Acrobat Reader to view., pdf, 1832.269 KB |
| Rights | http://www.ohsu.edu/library/etd_rights.shtml |
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