Highthroughput methods have been increasingly applied to catalyst screening, however, efforts to use these for enantioselective measures are still lacking. We propose to apply Fluorescence Resonance Energy Transfer (FRET) as a highthroughput screening method to fulfill such a purpose. This concept is applied to the desymmetrization of meso substrates. The meso compound will be equipped with a recognition element for catalyst binding, two different fluorescence donor molecules to distinguish between the chiral centres and also a fluorescence acceptor molecule to suppress fluorescence. Upon catalytic hydrolysis, the fluorescence acceptor molecules will be discharged into solution and thus can be detected by use of a spectrophotometer. As each donor molecule has a characteristic fluorescence emission wavelength, measuring the respective fluorescence intensities will ultimately allow for one to rapidly determine the enantiomeric excess. Efforts towards establishing this FRET based assay are discussed herein.*
*Please refer to dissertation for diagrams.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/27118 |
| Date | January 2006 |
| Creators | Chin, Ami Jun-Yee |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 227 p. |
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