Previous studies strongly suggested that RBP1 regulated E2F-dependent transcription. A previous pilot experiment also suggested that RBP1 exited the nucleus during muscle cell differentiation. / In this study, repeated approaches and attempts consistently supported the alternative hypothesis that RBP1 remained nuclear during muscle differentiation. A key role for RBP1 in muscle differentiation was repeatedly suggested using RNAi. Specifically, siRNA was used to 'knock down' RBP1. The inability of myoblasts to form myotubes without RBP1 was illustrated by an inability of RBP1-depleted cells to express myosin after appropriate muscle-differention medium was applied. / Further initial work suggested that RBP1's probable essential role in muscle differentiation was independent of RBP1's role in regulating E2F-dependent transcription.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.101707 |
| Date | January 2006 |
| Creators | Blank, Michael Warren. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biochemistry.) |
| Rights | © Michael Warren Blank, 2006 |
| Relation | alephsysno: 002585935, proquestno: AAIMR32820, Theses scanned by UMI/ProQuest. |
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