Proteins were isolated from acidic extracts and alkaline extracts of white kidney beans (Phaseolus vulgaris), navy beans (Phaseolus vulgaris) and baby lima beans (Phaseolus lunatus). The proteins were examined under a light microscope and a Scanning Electron Microscope. The isolated proteins had different microscopic structures; the structures depended on the conditions (pH and normality of extractant) of extraction. The various protein preparations were analysed for phytic acid, carbohydrates and citric acid. The nature of the protein-phytic acid reaction which takes place during the isolation of the proteins was investigated. The proteins that were isolated from the citric acid extracts and sodium hydroxide extracts of the beans were investigated by use of (a) electrophoretic analysis under acidic and under basic conditions in the absence and presence of dissociating agents, such as urea, 2-mercaptoethanol and sodium dodecyl sulphate, (b) ion-exchange chromatography and (c) gel-filtration chromatography.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68491 |
| Date | January 1979 |
| Creators | Alli, Inteaz. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Agricultural Chemistry and Physics) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000089650, proquestno: AAINK50376, Theses scanned by UMI/ProQuest. |
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