The structure and molecular forms of human placental type V collagen preparations containing (alpha)1(V), (alpha)2(V) and (alpha)3(V) chains were investigated. Crude human placental preparations containing native (alpha)1(V), (alpha)2(V) and (alpha)3(V) chains were resolved into two fractions by three independent nondenaturing methods (phosphocellulose chromatography, high performance ion exchange chromatography and ammonium sulphate fractionation). One fraction contained (alpha)1(V) and (alpha)2(V) chains in a 2:1 ratio and the other contained (alpha)1(V), (alpha)2(V) and (alpha)3(V) chains in a 1:1:1 ratio. The collagen in each fraction behaved as one homogeneous, native and distinct entity as determined by CD spectroscopy, resistance to trypsin/chymotrypsin digestion and electrophoresis under nondenaturing conditions. Trypsin cleaved collagens in both fractions at temperature near melting producing completely different fragmentation patterns, indicating that (alpha)1(V) and (alpha)2(V) chains in the two fractions were assembled in two different molecular forms. The collagens in the two fractions were also shown to differ in antigenic determinants in their native assemblies. (alpha)1(V) chains isolated from the two fractions were shown to differ in at least one antigenic determinant. / These data clearly demonstrate the existence of two distinct molecular forms of (alpha)1(V) (,2) (alpha)2(V) and (alpha)1(V) (alpha)2(V) (alpha)3(V) in human placenta.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71979 |
| Date | January 1984 |
| Creators | Niyibizi, Christopher. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000213529, proquestno: AAINL20840, Theses scanned by UMI/ProQuest. |
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