This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging.
| Identifer | oai:union.ndltd.org:columbia.edu/oai:academiccommons.columbia.edu:10.7916/D8HD82N6 |
| Date | January 2011 |
| Creators | Gatzogiannis, Evangelos George |
| Source Sets | Columbia University |
| Language | English |
| Detected Language | English |
| Type | Theses |
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