The regulation at the DNA/RNA level of the biosynthetic pathway of the antibiotic and mycotoxin, patulin, has yet to be elucidated. To lay the groundwork for further research into this area, a library of a genomic DNA sequences for Penicillium patulum was produced in the lambda phage, EMBL-3. Ninety induction-phase-specific clones were selected with a plus/minus screening technique which utilized labeled cDNA produced from mRNA isolated when the culture was uninduced and induced with respect to the patulin pathway. Five of these clones were partially characterized through restriction digest analysis, as well as, Northern dot blot analysis, which showed that some of the sequences are expressed shortly after induction of the pathway and some a few hours later. Eight subclones from the parent clones were produced in the plasmid, pUC18, and partially characterized, leading to a partial restriction map for two of the parent clones, which contain overlapping regions. / Source: Dissertation Abstracts International, Volume: 50-08, Section: B, page: 3452. / Major Professor: Robley J. Light. / Thesis (Ph.D.)--The Florida State University, 1989.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_78066 |
| Contributors | Kirsch, Paul Francis., Florida State University |
| Source Sets | Florida State University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | 161 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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