An infrared detector is constructed for use in High Pressure Liquid Chromatography. The detector uses a helium-neon laser which emits at 3.39 microns in the infrared as the source and a commercial lead selenide semiconductor as the detector. The detector when used in the flow-injection analysis mode can detect all classes of lipid: fatty acids, triglycerides, phospholipids, cholesterols and cholestryl esters. Chromatography using a normal phase column with separation into the lipid classes of cholesterol, cholestryl esters and triglycerides is performed. A purchased serum sample has been prepared and separation and quantitation of the 3 lipid classes is performed.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75894 |
Date | January 1987 |
Creators | Stokl, Caroline |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Doctor of Philosophy (Department of Chemistry.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: 000910032, proquestno: AAINL52227, Theses scanned by UMI/ProQuest. |
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