The biosynthesis of the antifungal, antiviral antiobiotic, sinefungin (1), produced by Streptomyces griseolus has been investigated. Precursor incorporation studies using (U-$\sp{14}$C), (5-$\sp3$H), (5-$\sp{13}$C) and (5-$\sp{13}$C, 5-$\sp{15}$N)-ornithine have shown that carbons 6$\sp\prime$-10$\sp\prime$ were derived from the intact incorporation of ornithine with retention of the C-5 nitrogen and with the loss of one of the protons from C-5 of ornithine.
The results of the feeding experiments of ($\sp{14}$C) and ($\sp3$H) labelled adenosine have indicated that the adenine moiety of adenosine was incorporated intact. Administration of adenosine labelled on the ribose moiety indicated that adenosine was incorporated into sinefungin with 50% loss of protons from C-5$\sp\prime$ of adenosine.
| Identifer | oai:union.ndltd.org:RICE/oai:scholarship.rice.edu:1911/16206 |
| Date | January 1989 |
| Creators | Arzu, Isidora Yvonne |
| Contributors | Parry, Ronald J. |
| Source Sets | Rice University |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | 94 p., application/pdf |
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