Thymidine dimers 16 and 18, connected by amide or N-methylamide linkages, have been prepared. The dimers were incorporated into normal strands of DNA by solid phase synthesis. Thermal denaturation studies, using complementary single-stranded RNA, indicated that these modifications caused no destabilization of the DNA-RNA duplex. / The block synthesis of amide-linked homotetramer 30 is described. The synthesis of the corresponding octamer could not be verified because of lack of solubility. One by one homologation was found to be a suitable method for the preparation of N-methylamide analogues. / Poly(ethylene glycol), covalently attached to the 3$ sp prime$ or 5$ sp prime$ end of amide-backbone thymidine homopolymers, was found to greatly increase their solubility. The poly(ethylene glycol) simultaneously served as a soluble solid support for the homologation reactions.$ sp*$ ftn$ sp*$Please refer to the dissertation for diagram.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.40145 |
| Date | January 1996 |
| Creators | Idziak, Irene |
| Contributors | Just, George (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001499923, proquestno: NN12389, Theses scanned by UMI/ProQuest. |
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