The primary goal of this study was to investigate the effect of the density of positively charged groups on the binding of bile acids by poly(acrylamide) resins with amine-containing pendent groups. For this purpose, lightly cross-linked poly(methyl acrylate) beads were functionalized by the reactions with diamines using two different methods, one of which eliminated additional cross-linking during the functionalization procedure. A comparison was made between the swelling characteristics, the isotherms for the binding of cholate anions, and the degree of cooperativity of these resins. Interestingly, the elimination of additional cross-linking not only improved the capacity of the resins to bind cholate anions but it also increased the selectivity for bile acid anions as well as their degree of positive cooperativity. The high degree of cooperativity minimally cross-linked poly(acrylamide) in binding bile acid anions probably indicates the existence of bile acid micelles or mixed micelles within the beads cavities. / To rationalize the effect of cross-linking density on the in vitro sorption efficiencies, a microstructure model for three dimensionally cross-linked beads was introduced. This model was developed according to the local degree of cross-linking and is based on the size of functional units and bile acids. / Furthermore, for the first time the effect of various organic counter-ions, such as propionate, acetate, benzoate, and salicylate on the in vitro sorption of cholate by poly(acrylamide) resins was studied. It was determined that the sorption efficiency can be improved significantly by replacing the chloride counter-ions with organic counter-ions of low relative affinity. It was demonstrated that functionalized poly(acrylamide) resins having acetate and propionate counter-ions possess a relatively better binding behavior for cholate anions than those resins with Cl$ sp-$ or I$ sp-$ counter-ions.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.23381 |
| Date | January 1995 |
| Creators | Asgari, Farajollah |
| Contributors | Brown, G. R. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001485621, proquestno: MM12155, Theses scanned by UMI/ProQuest. |
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