The biosynthesis of the antibiotic elaiomycin (1), produced by Streptomyces gelaticus, has been investigated. Precursor incorporation studies using (('13)C)- and {('3)H,('14)C}-labeled forms of serine have shown that carbons 2-4 of elaiomycin are derived from serine, with the hydroxymethyl carbon of serine being incorporated into C-4 of elaiomycin. An administration of (methyl-('13)C)-methionine has proven the methyl carbon of methionine to be the source of the O-methyl carbon of elaiomycin. (('13)C)- and {('14)C}-labeled precursor incorporation experiments indicate the novel introduction of C-2 of acetate into C-1 of elaiomycin. The administration of precursors labeled with contiguous ('13)C and ('15)N atoms has shown the oxygen-bearing nitrogen atom in elaiomycin to be derived from n-octylamine and the other nitrogen atom to be derived from serine.
| Identifer | oai:union.ndltd.org:RICE/oai:scholarship.rice.edu:1911/15846 |
| Date | January 1984 |
| Creators | MUELLER, JAY VINCENT |
| Source Sets | Rice University |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | application/pdf |
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