Dinoflagellates are a diverse group of protists, some of which are photosynthetic. The typical dinoflagellate chloroplast contains two proteins not found in any other photosynthetic eukaryote: peridinin-chlorophyll a-protein (PCP) and Form II ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). / Immunoelectron microscopic observations on the dinoflagellate Amphidinium carterae show that PCP is localized in the chloroplast stroma, not in the thylakoid lumen as previously postulated due to the presence of a putative lumen-targeting sequence. / Antisera against Form II Rubisco from bacterial (Rhodospirillum rubrum) and dinoflagellate (Symbiodinium sp.) sources were both shown to label the chloroplast and pyrenoid of A. carterae . Antiserum to Form I Rubisco from the haptophyte, alga Isochrysis galbana resulted in no labelling. Parallel experiments on the dinoflagellate Peridinium foliaceum, whose chloroplasts are all attributed to its diatom endosyrnbiont and on the diatom Phaeodactylum tricornutum revealed plastid pyrenoid labelling only with antiserum to Form I Rubisco.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20579 |
| Date | January 1998 |
| Creators | Jenks, Andrée. |
| Contributors | Gibbs, S. P. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001642139, proquestno: MQ44191, Theses scanned by UMI/ProQuest. |
Page generated in 0.0021 seconds