Our first objective was to compare deuterium incorporation and mass isotopomer distribution analysis in the measurement of in vivo cholesterol biosynthesis in humans. Twelve healthy subjects were recruited to participate in a 24 h stable isotope infusion study which mimicked temporal conditions typical of both deuterium incorporation and mass isotopomer distribution analysis techniques. Data suggest both deuterium incorporation and mass isotopomer distribution analysis accurately define cholesterol synthesis in humans when measured over a period of 24 h. / Our second objective was to examine the effects of weight loss through energy restriction upon human in vivo circulating cholesterol concentrations and synthesis using the deuterium incorporation methodology. Seven overweight subjects with a mean BMI of 30.6 +/- 1.6 kg·m-2 were recruited into a 2-phase prospective clinical trial including 3 mo on a weight stable American Heart Association Step I diet and 6 mo on an energy-reduced American Heart Association Step I diet. Data suggest that deuterium incorporation and mass isotopomer distribution analysis are equally effective methods for measuring cholesterol biosynthesis in humans, and that energy restriction resulting in modest weight loss effectively reduces endogenous cholesterol synthesis thus decreasing the risk of CVD development in obese and overweight men. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21542 |
| Date | January 1998 |
| Creators | Di Buono, Marco. |
| Contributors | Jones, Peter J. H. (advisor), Wykes, Linda J. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (School of Dietetics and Human Nutrition.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001657849, proquestno: MQ50757, Theses scanned by UMI/ProQuest. |
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