<div>
<p>Mammalian embryos undergo a dramatic amount of epigenetic remodeling
during the first week of development to establish the correct epigenetic status
to support the developmental program. SWI/SNF chromatin remodeling complexes
are multi-subunits complexes and utilize energy from ATP hydrolysis to modify
chromatin structure non-covalently. The collection of
subunits determines the identity of a given SWI/SNF chromatin-remodeling
complex, directs its activity, and dictate where that complex will act. The aims of this study were to 1) determine the requirement
of SNF5, a SWI/SNF core subunit found in BAF and PBAF complexes during preimplantation
porcine embryo development, 2) determine the requirement of BRD7, a PBAF
complex-specific subunit during preimplantation porcine embryo development, and
3) investigate the role of <i>CDH1</i>, a downstream gene regulated by ARID1A,
another subunit found exclusively in BAF complexes, in cleavage stage porcine
embryos. Our results indicate that the
differential requirement for each subunit during early embryo development.
Depletion of different subunits results in embryo arrest at distinct developmental
stage. Together, our data suggest the SWI/SNF chromatin remodeling complexes are
necessary for proper porcine embryo development and this requirement is
associated with the composition of the complex.</p>
</div>
<br>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/14378906 |
| Date | 07 May 2021 |
| Creators | Yu-Chun Tseng (10531823) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/Alteration_of_BRG1-_or_BRM-associated_factors_BAFs_components_of_SWI_SNF_chromatin_remodeling_complex_affects_preimplantation_porcine_embryo_development/14378906 |
Page generated in 0.0027 seconds