The aim of this diploma thesis was to determine, using gas chromatography, fatty acids in selected animal tissues. Specifically, the muscle, liver, pulmonary and adipose tissues were observed. A total of 32 piglets were divided into two groups with 16 pigs in each group. During 75 days of fattening, each group was given a feed mixture differing in the type of oil added. The experimental group was fed a basic feed mixture with the addition of 2,5 % fish oil, whilst the control group with the addition of 2,5 % palm oil. The animals were fed ad libitum. After slaughter, samples of selected tissues were taken and the process of lyophilisation was carried out. Using extraction method according to Hara, Radin (1978), lipids from lyophilized tissue samples were extracted. Fatty acids present within these lipids were then transformed into more volatile fatty acid methyl esters by the process of derivatization. These were then determined by gas chromatography and the results were statistically evaluated. The effect of fish oil in the feed mixture resulted in the increase of PUFA n-3 (P < 0.01) in all of the tested tissues. The addition of palm oil, on the other hand, had an effect on the content (P < 0.05) of SFA, MUFA in the tested tissues.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430581 |
| Date | January 2017 |
| Creators | Drábková, Michaela |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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