The principal aim of this study was to develop a new bed material suitable for gel exclusion chromatography, which would embrace all the desirable characteristics of
available materials used for this purpose and if possible improve on some of them. The two most important limitations of existing gel media concern their exclusion limits and rigidity. Agar and agarose have been found satisfactory for the separation of larger molecules including some viruses, but difficulties may arise at low gel concentrations due to the low mechanical strength of the granules. The dextran and polyacrylamide gels have in general a rigidity equal to that of agar and agarose, but cannot be prepared with such large pore sizes and are therefore limited to the separation of smaller molecules.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/32028 |
| Date | 07 April 2020 |
| Creators | Katz, Woolf |
| Publisher | Faculty of Health Sciences, Division of Medical Virology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Doctoral Thesis, Doctoral |
| Format | application/pdf |
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