by Suk Che Ho. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 86-88). / Abstract also in Chinese. / Abstract --- p.i / Acknowledgments --- p.iii / Dedication --- p.iv / Abbreviations --- p.v / Table of Contents --- p.vi / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Overview of Chinese Medicinal Herbs containing essential oils --- p.1 / Chapter 1.1.1 --- Introduction of Chinese Medicinal Herbs --- p.1 / Chapter 1.1.2 --- Chinese Medicinal Herbs containing essential oils --- p.2 / Chapter 1.2 --- Recognition of Chinese Medicinal Herbs --- p.2 / Chapter 1.2.1 --- Traditional method in recognition of Chinese Medicinal Herbs (CMH) --- p.2 / Chapter 1.2.2 --- Instrumental Methods for the recognition of CMH --- p.4 / Chapter 1.2.3 --- The use of GC and GC/MS on CMH --- p.4 / Chapter 1.3 --- Motivation and objective of this research --- p.5 / Chapter 1.3.1 --- Motivation --- p.6 / Chapter 1.3.2 --- Objective of this research --- p.7 / Chapter 1.4 --- Outline of the methodology and arrangement of the thesis --- p.8 / Chapter Chapter 2: --- Experimental Setup --- p.11 / Chapter 2.1 --- Reagents and materials --- p.11 / Chapter 2.1.1 --- Reagents and glassware --- p.11 / Chapter 2.1.2 --- Materials --- p.11 / Chapter 2.2 --- Sample pretreatment --- p.14 / Chapter 2.3 --- Extraction of essential oils from the herbal samples --- p.14 / Chapter 2.3.1 --- Traditional extraction methods for essential oils --- p.14 / Chapter 2.3.2 --- Extraction by hydrodistillation using Dean and Stark-type trap --- p.15 / Chapter 2.4 --- Results --- p.17 / Chapter 2.4.1 --- Comparison with literature data --- p.17 / Chapter 2.4.2 --- Reproducibility of the extraction --- p.17 / Chapter 2.4.3 --- Recovery test --- p.26 / Chapter 2.5 --- Discussion --- p.27 / Chapter Chapter3: --- Instrumental Analysis of the Essential Oils --- p.29 / Chapter 3.1 --- GC analysis --- p.29 / Chapter 3.1.1 --- Instrumentation --- p.29 / Chapter 3.1.2 --- Instrumental settings --- p.31 / Chapter 3.1.3 --- The use of GC in the analysis of essential oils --- p.31 / Chapter 3.1.3.1 --- Qualitative data --- p.31 / Chapter 3.1.3.2 --- Quantitative data --- p.33 / Chapter 3.1.3.3 --- Dilution strategy --- p.33 / Chapter 3.1.4 --- Results --- p.36 / Chapter 3.1.4.1 --- Precision test --- p.36 / Chapter 3.1.4.2 --- Linearity --- p.39 / Chapter 3.2 --- GC/MS analysis --- p.41 / Chapter 3.2.1 --- Instrumentation --- p.41 / Chapter 3.2.2 --- Instrumental settings --- p.42 / Chapter 3.2.3 --- The use of GC/MS in the analysis of essential oils --- p.43 / Chapter 3.2.3.1 --- Identification by GC/MS --- p.43 / Chapter 3.2.3.2 --- Abundance information --- p.43 / Chapter 3.2.4 --- Results --- p.44 / Chapter 3.2.4.1 --- Precision test --- p.44 / Chapter 3.2.4.2 --- Linearity --- p.46 / Chapter 3.2.4.3 --- Detection limit --- p.48 / Chapter 3.2.4.4 --- Chromatographic patterns of herbal samples obtained by GC/MS --- p.49 / Chapter 3.3 --- Discussion --- p.49 / Chapter Chapter 4: --- Development of a system for recognition --- p.52 / Chapter 4.1 --- Introduction --- p.52 / Chapter 4.2 --- Analysis of chromatographic patterns --- p.53 / Chapter 4.2.1 --- Extraction of “effective´ح peaks --- p.54 / Chapter 4.2.2 --- Extraction of “characteristic´ح peaks --- p.56 / Chapter 4.3 --- Library section --- p.60 / Chapter 4.3.1 --- Calculation of relative retention indices --- p.60 / Chapter 4.3.2 --- Normalization factors --- p.61 / Chapter 4.4 --- Matching section --- p.62 / Chapter 4.4.1 --- Overview of the matching method --- p.62 / Chapter 4.4.2 --- Input --- p.63 / Chapter 4.4.3 --- Matching strategy --- p.64 / Chapter 4.4.4 --- Matching algorithms --- p.64 / Chapter 4.4.4.1 --- "Matching with “characteristic"" peaks" --- p.64 / Chapter 4.4.4.2 --- Matching with “effective´ح peaks --- p.65 / Chapter 4.4.5 --- Calculation of similarity scores --- p.66 / Chapter 4.4.6 --- Output --- p.69 / Chapter Chapter 5: --- Performance of the proposed recognition system --- p.70 / Chapter 5.1 --- Recognition performance of the database --- p.70 / Chapter 5.1.1 --- Definition of similarity --- p.70 / Chapter 5.1.2 --- Performance test of the recognition method --- p.70 / Chapter 5.1.2.1 --- Candidates in the library file --- p.70 / Chapter 5.1.2.2 --- Unknown not found in the library file --- p.75 / Chapter 5.1.3 --- Information drawn from the scores --- p.77 / Chapter 5.1.3.1 --- Recognition of the unknown sample in terms of similarity --- p.77 / Chapter 5.1.3.2 --- Relationship between the herbal drugs --- p.79 / Chapter 5.2 --- Applicability of the proposed methodology --- p.80 / Chapter 5.3 --- Limitation of the proposed methodology --- p.81 / Chapter 5.4 --- Future prospect --- p.81 / Chapter Chapter 6: --- Conclusion --- p.83 / References --- p.86 / Appendices / Chapter A. --- Linearity of calibration graphs using GC --- p.A1 / Chapter B. --- Linearity of calibration graphs using GC/MS --- p.A3 / Chapter C. --- GC/MS chromatograms of the herbal samples --- p.A5 / Chapter D. --- "Relative retention times of “effective"" and ""characteristic"" peaks" --- p.A28
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322415 |
| Date | January 1998 |
| Contributors | Ho, Suk Che., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 88, 34 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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