Made available in DSpace on 2014-12-17T15:32:32Z (GMT). No. of bitstreams: 1
FernandoJON_TESE.pdf: 1862708 bytes, checksum: c0bcb5ceb1bfbf2e1312b265a0c10350 (MD5)
Previous issue date: 2013-09-13 / Periodontal disease is an infectious disease resulting from the immunoinflammatory
response of the host to microorganisms present in the dental biofilm which causes
tissue destruction. The objective of this study was to evaluate the
immunohistochemical expression of cyclophilin A (CYPA), extracellular matrix
metalloproteinase inducer (EMMPRIN) and matrix metalloproteinase 7 (MMP-7) in
human specimens of clinically healthy gingiva (n=32), biofilm-induced gingivitis
(n=28), and chronic periodontitis (n=30). Immunopositivity for CYPA, EMMPRIN and
MMP-7 differed significantly between the three groups, with higher percentages of
staining in chronic periodontitis specimens, followed by chronic gingivitis and healthy
gingiva specimens (p < 0.001). Immunoexpression of CYPA and MMP-7 was higher
in the intense inflammatory infiltrate observed mainly in cases of periodontitis.
Analysis of possible correlations between the immunoexpression of EMMPRIN,
MMP-7 and CYPA and between the expression of these proteins and clinical
parameters (probing depth and clinical attachment loss) showed a positive
correlation of CYPA expression with MMP-7 (r = 0.831; p < 0.001) and EMMPRIN (r
= 0.289; p = 0.006). In addition, there was a significant positive correlation between
probing depth and expression of MMP-7 (r = 0.726; p < 0.001), EMMPRIN (r = 0.345;
p = 0.001), and CYPA (r = 0.803; p < 0.001). These results suggest that CYPA,
EMMPRIN and MMP-7 are associated with the pathogenesis and progression of
periodontal disease / A doen?a periodontal ? uma entidade infecciosa que resulta da resposta
imuno-inflamat?ria do hospedeiro aos microrganismos presentes no biofilme
dent?rio, levando ? destrui??o tecidual. O prop?sito do presente estudo foi avaliar a
express?o imuno-histoqu?mica da ciclofilina A (CYPA), do indutor de
metaloproteinases da matriz extracelular (EMMPRIN) e da metaloproteinase da
matriz 7 (MMP-7) em esp?cimes humanos de gengiva clinicamente saud?vel (n=32),
gengivite induzida por biofilme dent?rio (n=28) e periodontite cr?nica (n=30). Foram
realizadas bi?psias das tr?s condi??es cl?nicas e feita a an?lise imuno-histoqu?mica
atrav?s da contagem total do n?mero de c?lulas positivas, correlacionando-a com
par?metros cl?nicos. A imunopositividade da CYPA, do EMMPRIN e da MMP-7
revelou diferen?a estatisticamente significativa entre os tr?s grupos, com maiores
percentuais de positividade nos esp?cimes de periodontite cr?nica, seguidos pelos
esp?cimes de gengivite cr?nica e de gengiva saud?vel (p < 0,001). Foi evidenciada
maior express?o de CYPA e MMP-7 nos grupos que tinham infiltrado inflamat?rio
mais intenso. Foram observadas correla??es das imunoexpress?es de EMMPRIN,
MMP-7 e CYPA, tanto entre si como com par?metros cl?nicos (profundidade de
sondagem e perda de inser??o cl?nica). Foram verificadas correla??es positivas
entre a express?o de CYPA e as express?es da MMP-7 (r = 0,831; p < 0,001) e do
EMMPRIN (r = 0,289; p = 0,006). Al?m disso, a profundidade de sondagem revelou
correla??o positiva, estatisticamente significativa, com as express?es de MMP-7 (r =
0,726; p < 0,001), EMMPRIN (r = 0,345; p = 0,001) e CYPA (r = 0,803; p < 0,001).
Esses resultados evidenciam que a CYPA, o EMMPRIN e a MMP-7 podem estar
associadas ? patog?nese e progress?o da doen?a periodontal
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17158 |
| Date | 13 September 2013 |
| Creators | N?brega, Fernando Jos? de Oliveira |
| Contributors | CPF:59539224420, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793681A0&dataRevisao=null, Silveira, Ericka Janine Dantas da, CPF:02869049420, http://lattes.cnpq.br/2186658404241838, Dantas, Euler Maciel, CPF:72172401404, http://lattes.cnpq.br/6454218688409728, Nonaka, Cassiano Francisco Weege, CPF:02781932426, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4133887J1&dataRevisao=null, Seabra, Eduardo Jos? Guerra, CPF:91566070406, http://lattes.cnpq.br/2882454174218035, Queiroz, L?lia Maria Guedes |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0019 seconds