The focus of this study was to identify the phenotype and genotype of three mutants of the green alga Chlamydomonas reinhardtii. These three mutants, all of the mating type plus, were created by random insertional mutagenesis, and subsequently screened for their inability to form zygotes when mixed with mating type minus gametes. To characterize individual phenotypes, the mutants were observed at each individual stage of the fertilization process, from agglutination through mating structure fusion and zygote formation. All three mutants showed normal progression of fertilization but failed to form fertilization tubules. Following phenotypic characterization, each mutant was subject to TAIL-PCR and DNA sequencing to determine the location of the insert. The location of the insert was determined in two of the three mutants. One mutant was defective in the actin-encoding gene (ida5) while the second was a possible insertion into a portion of mitochondrial DNA.
| Identifer | oai:union.ndltd.org:TCU/oai:etd.tcu.edu:etd-03162010-120401 |
| Date | 16 March 2010 |
| Creators | Roberts, Condred Curtis |
| Contributors | Michael J Misamore |
| Publisher | Texas Christian University |
| Source Sets | Texas Christian University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf, application/octet-stream |
| Source | http://etd.tcu.edu/etdfiles/available/etd-03162010-120401/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to TCU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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