Hydrilla verticillata is an invasive aquatic weed in the United States that has recently developed resistance to the herbicide fluridone due to point mutations in the pds gene. I amplified and sequenced the pds gene of 49 hydrilla samples from five Florida water bodies that vary in resistance to fluridone from none to very high. Genetic diversity within individuals was high with most individuals having 2-3 different pds alleles. I found two previously described resistance alleles and their occurrence across water bodies was broadly consistent with the observed levels of fluridone resistance. Fluridone resistance alleles were also cloned at higher frequencies within individuals than the susceptible alleles in water bodies with intermediate and high resistance to fluridone suggesting there are multiple copies of these alleles. Our study suggests that point mutations and allele copy number may both be responsible for fluridone resistance in hydrilla.
| Identifer | oai:union.ndltd.org:TCU/oai:etd.tcu.edu:etd-04052013-130818 |
| Date | 05 April 2013 |
| Creators | Tallent, Daniel Edward |
| Contributors | Dean A Williams, John D Horner, Amanda M Hale, Tamie Morgan |
| Publisher | Texas Christian University |
| Source Sets | Texas Christian University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.tcu.edu/etdfiles/available/etd-04052013-130818/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to TCU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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