Mucus secretion is the first-line of defence against the barrage of irritants inhaled into human lungs, but abnormally thick and viscous mucus results in many respiratory diseases. Investigation of processes underlying mucus pathology is hampered, in part, by lack of appropriate experimental tools for labeling and studying mucin granule secretion from live cells with high sensitivity and temporal resolution. Fluorescence spectra and fluorescence lifetime of AO measurements reveal significant changes due to aggregation, and this properties can be useful for determination of mucus expansion.\nIn particular report I introduce an original technique that enchances quality of the exocytotic process study in Calu 3 cells. Multi - pulse excitation significantly increases number of excited aggregates at low concentration of Acridine Orange that reveals possibility to monitor lower concentration than with classic single\npulse excitation. Increase of the signal strength increases sensitivity of measurements that is very important for the detection of small concentrations (up to nanomolars).
| Identifer | oai:union.ndltd.org:TCU/oai:etd.tcu.edu:etd-05132014-112308 |
| Date | 13 May 2014 |
| Creators | Shumilov, Dmytro |
| Contributors | Karol Gryczynski, Julian Borejdo, Bill Graham, Hana Dobrovolny, Waldek Zerda |
| Publisher | Texas Christian University |
| Source Sets | Texas Christian University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.tcu.edu/etdfiles/available/etd-05132014-112308/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to TCU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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