This study examined how the monoblast-like human histiocytic lymphoma cell line U937 can be induced by phorbol 12-myristrate 13-acetate (PMA) to undergo differentiation. In order to study the mechanism of action of CSF-1, a CSF-1 receptor gene (c-fms) was transfected into U937 cells. Exogenous CSF-1 treatment induced an autocrine response in this CSF-1 was determined and all events were shown to be time dependent. CSF-1 stimulation also enhanced proto-oncogene c-jun and c-myc gene expression. Complementary DNA coding for Jun or Fos was introduced into U937 cells by transfection. The transfection did not generate a high level of CSF-1 gene expression which suggests that Fos and Jun alone are insufficient to induce CSF-1 synthesis.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798317 |
| Date | 08 1900 |
| Creators | Liu, Mu-ya |
| Contributors | Wu, Edward Ming-chi, 1938-, Pirtle, Robert M., Masaracchia, Ruthann A., Gracy, Robert W., Jacobson, Myron, Benjamin, Robert C. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | x, 115 leaves : ill., Text |
| Rights | Public, Liu, Mu-ya, Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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