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Induced CSF-1 Production and its Effects on C-FMS Transfected Monoblastic U937 Cells

This study examined how the monoblast-like human histiocytic lymphoma cell line U937 can be induced by phorbol 12-myristrate 13-acetate (PMA) to undergo differentiation. In order to study the mechanism of action of CSF-1, a CSF-1 receptor gene (c-fms) was transfected into U937 cells. Exogenous CSF-1 treatment induced an autocrine response in this CSF-1 was determined and all events were shown to be time dependent. CSF-1 stimulation also enhanced proto-oncogene c-jun and c-myc gene expression. Complementary DNA coding for Jun or Fos was introduced into U937 cells by transfection. The transfection did not generate a high level of CSF-1 gene expression which suggests that Fos and Jun alone are insufficient to induce CSF-1 synthesis.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798317
Date08 1900
CreatorsLiu, Mu-ya
ContributorsWu, Edward Ming-chi, 1938-, Pirtle, Robert M., Masaracchia, Ruthann A., Gracy, Robert W., Jacobson, Myron, Benjamin, Robert C.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatx, 115 leaves : ill., Text
RightsPublic, Liu, Mu-ya, Copyright, Copyright is held by the author, unless otherwise noted. All rights

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