Abstract
The effect of tannic acid (TA), gallic acid (GA), propyl gallate (PA) and ellagic acid (EA) on DNA damage in human lymphocytes induced by food mutagens, 3-amino-1-methyl-5 H-pyrido (4,3-b)indole (Trp-P-2) and 2-amino-1-methyl-6-phenylimadazo (4,5-b)pyridine (PhIP), and/or H2O2 was evaluated using single-cell electrophoresis (comet assay). The toxicity of these tested compounds on lymphocytes was not found. These compounds did not cause DNA damage at lower concentration of 0.1-10 µg/ml. At a concentration of 100 µg/ml, TA and GA exhibited slight DNA damage, whereas PA and EA showed no DNA damage. TA and its related compounds decreased the DNA damage induced by Trp-P-2 or PhIP at a concentration of 0.1-10 µg/ml. Moreover, the inhibition of H2O2-induced DNA damage increased with increasing concentrations up to 10 µg/ml. DNA repair enzymes, endonuclease III (Endo III) and formamidopyrimidine-DNA glycoslase (FPG) were used to examine the levels of oxidised pyrimidines and purines in DNA damage induced by H2O2, respectively. All the compounds at 10 µg/ml can reduce the level of FPG sensitive sites. However, only EA inhibited the formation of EndoIII sensitive sites. The results indicate that these compounds can enhance lymphocyte resistance towards DNA damage induced by food mutagens or H2O2.
Keywords: Tannic acid; Human lymphocyte; Comet assay; Hydrogen peroxide
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0728103-133420 |
| Date | 28 July 2003 |
| Creators | Chu, Cheng-Chang |
| Contributors | none(¼B¥ò±d), none(Áé´º¥ú), none(³¯«T¾Ë), none(³¯®v¼y) |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-133420 |
| Rights | unrestricted, Copyright information available at source archive |
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