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Previous issue date: 2013-03-27 / Introduction : subjects with Down syndrome (DS) have an increased susceptibility to bacterial and viral infections and autoimmune diseases. These conditions are a major cause of morbidity and mortality in most cases. Thus, the objective of this study was to evaluate the profile of the immune response (innate and adaptive) of children with DS.
Methods : this was a case-control study. Patients with DS and controls were selected by a convenience sampling. Recruitment was conducted in a university hospital and a Center for Integration of Children with Special Needs (Kinder), both in the city of Porto Alegre. To evaluate the immune function was performed purification of human monocytes and human neutrophils, chemotaxis assay of human neutrophils, proliferation assay and immunophenotyping.
Results : this study included 13 (10/13 fem.) patients with DS and 6 (2/6 fem.) controls, with a mean age of 8.13 and 9.83 years respectively. When we measured neutrophil migration, there were no significant changes in the DS group. Moreover, analyzing the variables associated with the adaptive immune response, we observed a trend of lower percentages for CD4+ T lymphocyte and higher for CD8+ in the group with DS. When we analyzed the CD4+/CD8+ ratio, a significant difference was observed even considering the small sample size.
Conclusion : this study, together with the analysis of previous studies, suggests that patients with DS show a decreased CD4+/CD8+ ratio, which may explain the clinical picture of recurrent infections in these children / Introdu??o : sujeitos com s?ndrome de Down (SD) t?m uma maior suscetibilidade ?s infec??es virais e bacterianas e a doen?as autoimunes, que s?o as principais causas de morbimortalidade na maioria dos casos. Dessa forma, o objetivo do presente estudo foi avaliar o perfil da resposta imunol?gica (inata e adaptativa) de crian?as com SD.
M?todos : este foi um estudo de caso-controle. Os pacientes com SD e os controles foram selecionados por uma amostragem de conveni?ncia. O recrutamento foi realizado em um hospital universit?rio e em um Centro de Integra??o de Crian?as Especiais (Kinder), ambos na cidade de Porto Alegre. Para avaliar a fun??o da resposta imune, foram realizados purifica??o de mon?citos e de neutr?filos humanos, ensaio de quimiotaxia de neutr?filos humanos e imunofenotipagem.
Resultados : neste estudo, foram inclu?dos 13 (10/13 fem.) pacientes com SD e 6 (2/6 fem.) controles normais, com m?dias de idades de 8,13 e 9,83 anos respectivamente. Quando estudamos a capacidade de migra??o dos neutr?filos, n?o foram observadas altera??es significativas no grupo com SD. Por outro lado, quando analisamos as vari?veis associadas ? resposta imune adaptativa, observamos uma tend?ncia a valores percentuais menores de linf?citos T CD4+ e maiores de CD8+ para o grupo com SD. Quando analisamos a rela??o CD4+/CD8+, foi observada diferen?a significativa mesmo com um pequeno n?mero amostral.
Conclus?o : esse estudo, em conjunto com a an?lise de estudos pr?vios, sugere que os pacientes com SD apresentam uma taxa CD4+/CD8+ diminu?da, o que pode justificar o quadro cl?nico de infec??es recorrentes nessas crian?as
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1417 |
| Date | 27 March 2013 |
| Creators | Kautzmann Filho, Artur |
| Contributors | Pinto, Leonardo Ara?jo |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina/Pediatria e Sa?de da Crian?a, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3098206005268432148, 500, 600, -8624664729441623247 |
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