Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dL-lactic-coglycolic ( PLGA) functionalized with polyethylene glycol ( PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below 24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/622432 |
| Date | 25 November 2016 |
| Creators | Gutiérrez-Valenzuela, Cindy, Guerrero-Germán, Patricia, Tejeda-Mansir, Armando, Esquivel, Reynaldo, Guzmán-Z, Roberto, Lucero-Acuña, Armando |
| Contributors | Univ Arizona, Dept Chem & Environm Engn |
| Publisher | MDPI AG |
| Source Sets | University of Arizona |
| Language | English |
| Detected Language | English |
| Type | Article |
| Rights | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0). |
| Relation | http://www.mdpi.com/2076-3417/6/12/364 |
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