The methods of denaturing gradient gel electrophoresis (DGGE) and DNA sequencing were used to analyze the ribotypes of microbial communities associated with corals. Both healthy and diseased coral of different species were collected at three locations off the southern coast of Taiwan. Ribotyping results suggested that the microbial communities were diverse. The microbial community profiles, even among the same species of corals from different geographical locations or different times, differ significantly. The coral-associated bacterial communities contain many bacteria common to the habitants of various invertebrates. One ribotype presented on the Black Disease coral tissue is closely related to the Clostridium sp. previously identified from White Plaque-diseased and BBD corals. This Clostridium sp. may be relatived to coral diseased, as this species was also found in many types of diseased coral. The other one ribotype presented on our healthy and diseases coral is closed to denitrifying bacterium Pseudovibrio sp. which had been previously discovered in Kenting National Park Nanwan Bay as a new marine, facultative anaerobic bacterium. However, some bacteria were unexpected. The presence of some unusual species, such as Staphylococcus, Legionella and soil bacteria, associated with corals that were likely the results of human activities. Human activities, such as active fishing and tourism industries in the region might have all contributed to the change in bacterial communities and the death of coral colonies around the region.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0616110-184530 |
| Date | 16 June 2010 |
| Creators | Chiu, Su-fen |
| Contributors | Chi-Hsin Hsu, Jimmy Kuo, Chung-Lung Cho, Chan-Shing Lin, Jong-Kang Liu, Lee-Shing Fang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616110-184530 |
| Rights | not_available, Copyright information available at source archive |
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