Bats and birds are known to be the reservoirs of a number of zoonotic diseases. The
capacity of flight and the diversity of these animals may make them special in maintaining
and disseminating diverse viruses leading to instances of emerging zoonoses. In particular
bats are increasingly recognized to be reservoirs of a wide range of viruses, including Nipah,
Ebola and severe acute respiratory syndrome (SARS) coronaviruses. In most instances these
viruses appear to establish long-term persistence in bats.
In this thesis I report the identification of novel astroviruses from different
insectivorous species of apparently healthy bats sampled in Hong Kong and in 11 provinces
of Mainland China with high positive rates. Astroviruses are important causes of diarrhea in
many animal species, including humans. This study revealed a remarkably high genetic
diversity of bat astroviruses, which form novel distinct phylogenetic groups in the genus
Mamastrovirus. Evidence for varying degrees of host restriction for bats astroviruses has
been found. The finding of diverse astroviruses in Miniopterus bats captured within a single
cave habitat in Hong Kong illustrates a very unusual virus host relationship between
astroviruses and these bats.
Surveillance of astroviruses in rodents, the only mammal with species numbers
surpassing that of bats, has revealed a novel astrovirus in only 1.6 % of the faecal samples of
urban brown rat (Rattus norvegicus) in Hong Kong in marked contrast with the prevalence
and diversity of astroviruses in bats. Rat astrovirus was phylogenetically related to human
astroviruses MLB1 which was detected from clinical samples from diarrhoeal patients in
Hong Kong in this study. The unusually high positive rates of astroviruses in bats have been
again highlighted.
Avastroviruses were detected in 7.1% of the aquatic wild bird samples. Avastrovirus
have also been detected in doves in Hong Kong, pond herons and a less whistling duck in
Cambodia. A phylogenetic analysis of these novel astroviruses together with other previously
known astroviruses revealed that avastrovirus can be divided into 3 monophyletic groups. On
the other hand, avian coronaviruses was detected in 12.5% of the aquatic wild bird samples.
Phylogenetic analysis of these avian coronaviruses has led us to suggest taxonomic separation
of these viruses into two groups as gammacoronaviruses and deltacoronaviruses. Frequent
interspecies transmissions of gammacoronaviruses between duck species were demonstrated.
Analysis of the avian viral sequences and host mitochondrial DNA sequences suggested that
some coronaviruses may have coevolved with birds from the same order.
With the discoveries of coronaviruses and astroviruses in mammalian and birds, we
now have a better understanding on the diversity and ecology of these two virus families in
wildlife. These findings provide new insights into the ecology and evolution of these viruses
in nature and have revealed possible inter-species transmissions of these viruses. The role of
bats as a reservoir of viruses with potential to pose zoonotic threats to human health was also
reinforced. Studies of the virus ecology in wildlife as demonstrated in this thesis will help
formulating better strategies for controlling emerging diseases in the future. / published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy
| Identifer | oai:union.ndltd.org:HKU/oai:hub.hku.hk:10722/174531 |
| Date | January 2011 |
| Creators | Chu, Ka-wing., 朱嘉永. |
| Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
| Source Sets | Hong Kong University Theses |
| Language | English |
| Detected Language | English |
| Type | PG_Thesis |
| Source | http://hub.hku.hk/bib/B47849733 |
| Rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works., Creative Commons: Attribution 3.0 Hong Kong License |
| Relation | HKU Theses Online (HKUTO) |
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