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The effect of maturity and crop load on the browning and concentration of phenolic compounds of Thompson Seedless and Regal Seedless

Thesis (MScAgric)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Thompson Seedless and Regal Seedless are two white seedless table grape
cultivars widely produced in South Africa. Both cultivars are susceptible to berry
browning, especially Regal Seedless. Browning leads to annual financial losses for
table grape growers. Although a correlation between harvest maturity and the
occurrence of browning seems to exist, it is still unclear whether maturity levels are
the actual contributing factor. The aim of the study was to establish if harvest
maturity and crop load could influence the occurrence of browning of both cultivars.
The impact of harvest maturity and crop load on phenolic compound concentration in
the berry skin of both cultivars was also investigated. Total external browning of
Regal Seedless and Thompson Seedless occurred in much higher percentages than
internal browning. Regal Seedless showed a tendency to decreased total external
browning with harvest maturity. The main reason for this is that net-like browning,
which is the greatest contributor to total external browning, decreased with harvest
maturity, in all three seasons. External browning of Thompson Seedless increased
with harvest maturity in both seasons. Contact browning was the greatest contributor
to total external browning of Thompson Seedless. Crop load did not significantly
influence berry browning of Regal Seedless or Thompson Seedless grapes. The
flavan-3-ol concentration (catechin, epicatechin, procyanidin B1 and procyanidin B2)
in Regal Seedless generally increased with harvest maturity, whereas in Thompson
Seedless the general tendency was a decrease in the flavan-3-ol concentration with
harvest maturity. The development of phenolic compound concentration with
maturity could not be correlated with the occurrence of berry browning. Crop load did
not affect flavan-3-ol concentration. When the flavan-3-ol concentration of Regal
Seedless and Thompson Seedless were compared at different harvest maturities the
concentrations of flavan-3-ols were clearly much higher in the skin of Regal Seedless than in the skin of Thompson Seedless (for both the 2008 & 2009
seasons). Comparison of the browning incidence with harvest maturity for these two
cultivars (see above) clearly reveals that external browning of Regal Seedless
occurred in much higher percentages than on Thompson Seedless. Regal Seedless
had much higher levels of external browning than Thompson Seedless. The
concentration of flavan-3-ols in the skin of white seedless cultivars may be an
indication of the cultivar’s susceptibility to external browning. / AFRIKAANSE OPSOMMING: Thompson Seedless en Regal Seedless is twee wit pitlose tafeldruif kultivars wat
ekstensief in Suid-Afrika verbou word. Verbruining kan ‘n probleem wees by beide
kultivars, spesifiek Regal Seedless. Die faktore wat aanleiding gee tot verbruining is
nog nie duidelik bepaal nie. Alhoewel dit lyk of daar ‘n korrelasie tussen
rypheidsgraad van die oes en verbruining kan wees is dit steeds onduidelik of
oesrypheidsvlakke die werklike oorsaak van verbruining is. Die doel van die studie
was om vas te stel of die rypheidsgraad van die oes en oeslading verbruining van
beide kultivars kan beïnvloed. Die effek van oes rypheidsgraad en oeslading op
konsentrasie van fenoliese verbindings in die korrelskil van beide kultivars is ook
ondersoek. Totale eksterne verbruining van Regal Seedless en Thompson Seedless
het in baie hoër persentasies voorgekom as interne verbruining. Daar was ‘n
tendens by Regal Seedless dat totale eksterne verbruining verminder het soos die
oes ryper geraak het as gevolg van netagtige verbruining, wat die grootste bydrae tot
totale eksterne verbruining veroorsaak het. Netagtige verbruining se voorkoms het
verminder oor al drie seisoene. Eksterne verbruining van Thompson Seedless het
toegeneem met oes rypheid in beide seisoene. Kontak verbruining het grootste
byrdae gelewer tot totale eksterne verbruining van Thompson Seedless. Oeslading
het nie ‘n betekenisvolle invloed op verbruining van Regal Seedless en Thompson
Seedless gehad nie. Die flavan-3-ol (katesjien, epikatesjien, prosianidien B1 en
prosianidien B2) konsentrasie van Regal Seedless het met oes rypheid toegeneem.
By Thompson Seedless was daar ‘n afname in die flavan-3-ol konsentrasie met oes
rypheid. Daar was geen korrrelasie tussen die konsentrasie van fenoliese verbinding
en die voorkoms van verbruining vir beide kultivars. Oeslading het nie ‘n
betekenisvolle effek op die konsentrasie van fenoliese verbindings gehad nie.
Vergelyking van die flavan-3-ol konsentrasie van Regal Seedless en Thompson
Seedless by verskillende rypheidsgrade wys dat die konsentrasie baie hoër in die korrel skil van Regal Seedless as in die van Thompson Seedless (vir beide 2008 &
2009 seisoene). Die vergelyking van die voorkoms van verbruining met oesrypheid
van beide kultivars wys duidelik dat eksterne verbruining van Regal Seedless in baie
hoër persentasies voorkom as in Thompson Seedless. Flavan-3-ol konsentrasie in
die skil van wit pitlose kultivars kan ‘n aanduiding wees van die kultivar se moontlike
risiko vir die voorkoms van eksterne verbruining.

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/95886
Date12 1900
CreatorsKamfer, De Witt
ContributorsRaath, Pieter, Stellenbosch University. Faculty of AgriSciences. Dept. of Viticulture and Oenology.
PublisherStellenbosch : Stellenbosch University
Source SetsSouth African National ETD Portal
Languageen_ZA
Detected LanguageEnglish
TypeThesis
Format93 p. : ill.
RightsStellenbosch University

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