Cryopreservation in suspension is commonplace for a variety of cell types.
However, cryopreservation of adherent cells has achieved limited success. This research aimed to cryopreserve adherent nerve cell networks in vitro in a manner that preserved network morphology and physiology. Successful implementation would enable long term storage of adherent neuronal networks on microelectrode arrays and on-demand access for use in pharmacological and toxicological testing. Based upon morphological assessments, excellent post-thaw preservation was obtained and post-thaw cultures survived in a transitional medium for up to 3.5 hours. However, transitions to native culture medium post-thaw presented difficulties, ultimately resulting in necrosis. A discussion of methods to supplement the current research and increase post-thaw viability is included in the thesis.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc12213 |
| Date | 12 1900 |
| Creators | Webb, Veronica Fine |
| Contributors | Gross, Guenter W., Fuchs, Jannon L., Huggett, Duane B. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | Text |
| Rights | Public, Copyright, Webb, Veronica Fine, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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