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Effects of cytokinin and cations on greening process in excised cucumber cotyledons.

Pak-chung Leung. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 151-158). / Abstract --- p.i / Acknowledgments --- p.iii / Table of contents --- p.iv / List of Figures --- p.viii / List of Tables --- p.ix / List of Abbreviations --- p.xiv / Chapter / Chapter 1. --- General Introduction --- p.1 / Chapter 2. --- Literature Review: Greening process in higher plants --- p.4 / Chapter 3. --- Greening Bioassay in Excised Cucumber Cotyledons / Introduction --- p.13 / Materials and Methods / Plant material --- p.14 / Measurement of Ch1 synthesizing activity in excised cotyledons of different ages --- p.14 / Measurement of Ch1 accumulation in excised cotyledons after various pretreatment periods --- p.15 / Measurement of Ch1 accumulation in excised cotyledons under different light intensities and temperatures --- p.15 / Greening curve --- p.16 / Results and Discussion / Effect of seedling age on Ch1 accumulation --- p.16 / Effect of pretreatment period on Ch1 accumulation --- p.18 / Effects of light intensity and temperature on Ch1 accumulation --- p.18 / Effect of shaking (for oxygen replenishment) on Ch1 accumulation --- p.19 / Greening curve --- p.20 / Greening bioassay in excised cucumber cotyledons --- p.20 / Chapter 4. --- Regulation of Chlorophyll Accumulation in Excised Cucumber Cotyledons / Introduction --- p.27 / Materials and Methods / Materials --- p.28 / Seed germination --- p.28 / Harvesting and pretreatment --- p.28 / Illumination and Ch1 determination --- p.29 / Results / Effect of BA on Ch1 accumulation --- p.29 / Effect of Na2EGTA on Ch1 accumulation --- p.30 / "Effect of Na+, Ca2+ and K+ on Ch1 accumulation" --- p.32 / Effect of duration of pretreatment with Na+ , Ca2+ and K+ on Ch1 accumulation --- p.34 / Effect of verapamil and Ca ionophore A23187 on Chl accumulation --- p.36 / Effect of TFP on Chl accumulation --- p.37 / Discussion / Effect of BA on Chl accumulation --- p.38 / "Effect of Na+, Ca2+ and K+ on Chl accumulation" --- p.39 / Demonstration of the involvement of Ca2+ in Chl accumulation --- p.43 / Chapter 5. --- The Calcium Effect on Benzyladenine-induced Stimulation of Chlorophyll Accumulation in Excised Cucumber Cotyledons / Introduction --- p.63 / Materials and Methods / Materials --- p.64 / Plant growth and treatment --- p.64 / Pretreatment experiment --- p.64 / Sequence experiment --- p.65 / Results / Pretreatment experiment --- p.65 / Sequence experiment --- p.66 / Discussion --- p.68 / Chapter 6. --- Regulation of 5-Aminolevulinic Acid and Chlorophyll Accumulation in Levulinic Acid-treated Cotyledons / Introduction --- p.80 / Materials and Methods / Materials --- p.81 / Plant growth and treatment --- p.81 / Chl determination and ALA assay --- p.82 / Measurement of ALA and Chl accumulation in LA-treated cotyledons --- p.83 / ALA accumulation curve --- p.83 / Measurement of Chl accumulation in 50 mM NaCl-treated cotyledons --- p.84 / "Measurement of ALA and Chl accumulation in BA-, cations-, Na2EGTA-, verapamil- and TFP-pretreated cotyledons" --- p.85 / Results / Concentrations effect of LA on ALA and Chl accumulation --- p.85 / ALA accumulation curve --- p.86 / Effect of 50 mM NaCl on Chl accumulation --- p.87 / Effect of BA on ALA and Chl accumulation in LA- treated cotyledons --- p.90 / "Effects of Na+, Ca2+ and K+ on ALA and Chl accumulation in LA-treated cotyledons" --- p.91 / "Effects of Na2EGTA, verapamil and TFP on ALA and Chl accumulation in LA-treated cotyledons" --- p.92 / Discussion / Measurement of ALA accumulation in LA-treated cotyledons --- p.93 / Effect of BA on ALA and Chl accumulation in LA-treated cotyledons --- p.96 / "Effects of Na+, Ca2+ and K+ on ALA and Chl accumulation in LA-treated cotyledons" --- p.98 / Demonstration of the involvement of Ca2+ in ALA accumulation --- p.99 / Chapter 7. --- Regulation of 5-Aminolevulinic Acid Accumulation in Isolated Developing Chloroplasts / Introduction --- p.123 / Materials and Methods / Materials --- p.124 / Plant materials --- p.124 / Chloroplast isolation --- p.125 / Incubation conditions --- p.126 / ALA assay --- p.127 / Measurement of ALA accumulation in LA-treated isolated developing chloroplasts --- p.128 / "Measurement of ALA accumulation in BA-, cations-, Na2EGTA-, verapamil- and TFP-treated isolated developing chloroplasts" --- p.128 / Results / Time course study of Chl accumulation in intact greening cotyledons of different ages --- p.129 / Measurement of ALA synthesizing activity in isolated developing chloroplasts incubated in the dark and light --- p.130 / Concentrations effect of LA on ALA accumulation in isolated developing chloroplasts --- p.130 / Effects of BA and the cations on ALA accumulation in isolated developing chloroplasts --- p.131 / "Effect of Na2EGTA, verapamil and TFP on ALA accumulation in isolated developing chloroplasts" --- p.132 / Discussion / Light regulation of ALA synthesizing activity in isolated developing chloroplasts --- p.132 / Regulation of ALA accumulation in isolated developing chloroplasts --- p.134 / Chapter 8. --- Conclusion --- p.147 / References --- p.151

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318194
Date January 1994
ContributorsLeung, Pak-chung., Chinese University of Hong Kong Graduate School. Division of Biology.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xv, 158 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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