by Chan Ngar Lei Annie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 90-102. / ACKNOWLEDGEMENTS --- p.i / ABSTRACT --- p.ii / TABLE OF CONTENTS --- p.iv / LIST OF FIGURES --- p.vii / LIST OF TABLES --- p.ix / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 2. --- LITERATURE REVIEW --- p.5 / Chapter 2.1 --- Economic and Biotechnological Significance of Lentinus edodes --- p.5 / Chapter 2.1.1 --- General Review --- p.5 / Chapter 2.1.2 --- Nutritional and Medicinal Values --- p.7 / Chapter 2.1.3 --- Lignocellulose Degradation and Utilization --- p.9 / Chapter 2.2 --- Biological Background --- p.11 / Chapter 2.2.1 --- Life Cycle --- p.11 / Chapter 2.2.2 --- Patterns of Sexuality --- p.12 / Chapter 2.3 --- Genetic Improvement of Lentinus edodes --- p.16 / Chapter 2.3.1 --- Introduction --- p.16 / Chapter 2.3.2 --- Mutagenic Agents --- p.17 / Chapter 2.3.3 --- Genetic Markers and Strain Improvement --- p.20 / Chapter 3. --- MATERIALS AND METHODS --- p.24 / Chapter 3.1 --- Biological Materials --- p.24 / Chapter 3.2 --- Media --- p.25 / Chapter 3.2.1 --- Complete Medium (CM) --- p.25 / Chapter 3.2.2 --- Complete Medium with Yeast Extract (CM with Y.E.) --- p.25 / Chapter 3.2.3 --- Complete Fruiting Medium (CF) --- p.25 / Chapter 3.2.4 --- Complete Migration Medium (CMM) --- p.25 / Chapter 3.2.5 --- Minimal Medium (MM) --- p.25 / Chapter 3.2.6 --- Carboxymethyl Cellulose - Leatham Medium (CMC - Leatham) --- p.26 / Chapter 3.2.7 --- Potato Dextrose Agar (PDA) --- p.26 / Chapter 3.3 --- Anti-metabolites for Screening of Resistant Mutants --- p.27 / Chapter 3.4 --- Characterization of Monokaryons --- p.28 / Chapter 3.4.1 --- Isolation of Monosporous Mycelia --- p.28 / Chapter 3.4.2 --- Assessment of Mycelial Growth --- p.28 / Chapter 3.4.3 --- Determination of Mating Type of Monosporous Mycelia --- p.28 / Chapter 3.5 --- Mutagenesis and Isolation of Mutants --- p.29 / Chapter 3.5.1 --- Effects of Homogenization on Growth --- p.29 / Chapter 3.5.2 --- Determination of the Ultraviolet Irradiation Killing Curve --- p.29 / Chapter 3.5.3 --- Isolation of High Temperature Tolerant Strains --- p.30 / Chapter 3.5.4 --- Isolation of Auxotrophic Mutants --- p.30 / Chapter 3.5.5 --- Isolation of Anti-metabolite Resistant Mutants --- p.33 / Chapter 3.6 --- Characterization of Anti-metabolite Resistant Mutants --- p.33 / Chapter 3.6.1 --- "Measurements of Growth Rate, Anti-metabolites Resistance and Osmotic Sensitivity" --- p.33 / Chapter 3.6.2 --- Determination of Dominance Relationships --- p.34 / Chapter 3.6.3 --- Detection of Extracellular Enzymes --- p.34 / Chapter 4. --- RESULTS --- p.36 / Chapter 4.1 --- Radial Growth Rate of Monosporous Cultures --- p.36 / Chapter 4.2 --- Mating Reactions in Lentinus edodes --- p.36 / Chapter 4.3 --- Effects of Homogenization on Growth of L. edodes --- p.46 / Chapter 4.4 --- Determination of Ultraviolet (UV) Killing Curve --- p.46 / Chapter 4.5 --- Selection of High Temperature Tolerant Strains --- p.50 / Chapter 4.6 --- Auxotrophic Mutants Isolation and Identification --- p.50 / Chapter 4.7 --- Selection of Anti-metabolite Resistant Mutants --- p.52 / Chapter 4.8 --- Characterization of Cycloheximide Resistant Mutants V --- p.63 / Chapter 4.8.1 --- "Growth Rate, Anti-metabolite Resistance and Osmotic Sensitivity of Cycloheximide Resistant Mutants" --- p.63 / Chapter 4.8.2 --- Dominance Tests for Cycloheximide Resistant Strains --- p.66 / Chapter 4.8.3 --- Detection of Extracellular Enzymes --- p.73 / Chapter 5. --- DISCUSSION --- p.78 / Chapter 5.1 --- Mating Reactions in Lentinus edodes --- p.78 / Chapter 5.2 --- Isolation of Auxotrophic and Anti-metabolites Resistant Mutants --- p.81 / Chapter 5.3 --- Characterization of Cycloheximide Resistant Mutants of Lentinus edodes --- p.84 / CONCLUSION --- p.88 / REFERENCES --- p.90
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318603 |
| Date | January 1990 |
| Contributors | Chan, Ngar Lei., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 102 leaves ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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