The function of CABP1, a novel cAMP-binding protein in Dictyostelium discoideum, has been investigated. Previously, it has been shown that the molecular weight of CABP1 varies in different strains and that this variation is correlated with differences in response to cAMP. Sequence analysis showed that compared to its counterpart in strain AX2, the gene encoding CABP1 of strain V12M2 is missing 27 bp. AX2 transformants expressing the capA gene of V12M2 exhibited no change in phenotype, suggesting that CABP1 is not involved in the features examined or that the V12M2 variant is recessive. Disruption of capA by homologous recombination was attempted with no success, suggesting that this gene might be essential. Proteins interacting with CABP1 have been identified by immunoprecipitation using specific antibodies, CABP1 was also found to copurify with annexin VII, a calcium-dependent phospholipid-binding protein, suggesting that CABP1 and annexin VII might interact.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56646 |
| Date | January 1992 |
| Creators | Bonfils, Claire |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001312717, proquestno: AAIMM80420, Theses scanned by UMI/ProQuest. |
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