The diploma thesis was aimed to explain the role of cytokinins in regulation of cotyledonary bud outgrowth in pea (Pisum sativum L.). Decapitation of the apex and subsequent application of IAA lanolin paste containing also BAP caused increased expression of genes PsDRM1 and PsBRC1 associated with dormancy. It suggests that cytokinins may have effect on the amount of transported auxin in the major stem. Application of cytokinin to the cotyledonary buds of intact plants activated their outgrowth. Imunolocalization showed polarization of PIN1 proteins in the treated buds, on contrary to untreated buds. It means that cytokinin directly influenced polarization of PIN1 proteins and the subsequent canalization of the polar auxin transport.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430511 |
| Date | January 2017 |
| Creators | Vetter, Martin |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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