Magister Pharmaceuticae - Mpharm / Cancer is a major health problem worldwide and killing millions of people each year. The use
of natural products as chemotherapeutic agents is well established, however, many of the
currently available drugs are associated with undesirable side effects and high toxicity.
Furthermore, the development of drug resistant cancers makes the search for anticancer lead
compounds a priority. In this study a library of prefractionated marine sponge extracts was
established and used to prioritise samples for isolation of bioactive metabolites. From the
generated library, two of the sponges of genera Ircinia sp. and Latrunculid sp. resulted in
isolation of furanosesterterpenes (7E,12Z,20Z,18S-variabilin) and pyrroloiminoquinone
(tsitsikammamine A and tsitsikammamine N-18 oxime) alkaloids respectively. The structures
of these compounds were elucidated by analysis of 1D and 2D NMR data. These compounds
displayed moderate to potent cytotoxicity against MCF-7, PC-3, U-87 and HEK-293 cells lines
through apoptosis, with lack of selectivity for cancer cell lines.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uwc/oai:etd.uwc.ac.za:11394/6447 |
| Date | January 2018 |
| Creators | Lerata, Mookho Sylvia |
| Contributors | Denzil Beukes |
| Publisher | University of the Western Cape |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Rights | University of the Western Cape |
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