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DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers

The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc278947
Date08 1900
CreatorsChiu, Angela Chen-Yen
ContributorsShanley, Mark Stephen, Benjamin, Robert C., O'Donovan, Gerard A.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 74 leaves: ill., Text
RightsPublic, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Chiu, Angela Chen-Yen

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